Cell Technologies Megalaboratory operating on the basis of the Department of Chemistry and Technology of Biologically Active Compounds, Medical and Organic Chemistry named after N.A. Preobrazhensky

Cell Technologies Megalaboratory, opened at the Lomonosov Institute of Fine Chemical Technologies of RTU MIREA, provides for the training of bachelors, masters and graduate students in preclinical studies of pharmacologically active substances and drugs.

The main areas of work of the Megalaboratory:

Assessment of antitumor activity of photosensitizers and combination drugs based on them;;
Determination of the effectiveness of therapeutic nucleic acid delivery systems
Study of the effect of anti-inflammatory drugs based on allosteric lipoxygenase inhibitors;
Study of intracellular localization of drugs.

Skills acquired while working in the Megalab

By carrying out research work related to assessing the effect of biologically active compounds, students and graduate students of RTU MIREA receive the necessary skills to work with eukaryotic cells:

Maintaining the viability of cell lines and cell passaging;
Preparation of nutrient media, subculture and cultivation of eukaryotic cells;
Maintaining aseptic conditions in clean laboratory rooms.

A special place in the Megalaboratory belongs to an instrument complex that makes it possible to assess the activity and intracellular distribution of future drugs.

Students studying at RTU MIREA will be able to master:

Colorimetric methods for assessing the metabolic activity of cells;
Fluorescent and confocal microscopy methods for studying the intracellular distribution of substances;
Methods for determining the status of eukaryotic cells by studying the morpho-functional state of individual cells based on measuring optical properties.

Megalaboratory equipment:

Nexcope NIB620FL Biological inverted microscopes and Nexcope NIB900optical confocal system ;
BMB-II-“Laminar-S” Laminar microbiological safety boxes ~ Wiggens multi-gas CO2 incubators;
Alphavita Low temperature freezers;
Multimodal plate reader FeyondA-300 and automatic cell counter RWD Life Science;
General laboratory equipment: cryocentrifuges, microcentrifuges, shakers, automatic dispensers.

In the Cell Technologies Megalab, employees of the Department of Chemistry and Technology of Biologically Active Compounds, Medical and Organic Chemistry named after N.A. Preobrazhensky conduct initial preclinical trials of pharmacologically active substances, which can significantly speed up the process of developing innovative drugs.